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Luciana Maria Silva Lopes

Fundação Ezequiel Dias (FUNED)

Genetics and Biotechnology Studies in Cancer

Photo of Luciana Maria Silva Lopes

Associated Team:

  • Milene Pereira Moreira – Funed Server, Researcher
  • Bianca Nataliene Carvalho de Camargos - Master's Student PPG Biotec Funed, Scholar
  • Julia Guerra Vieira de Sousa - Master's Student PPG Biotec Funed, Scholar

Other products related to the Network:

  • Master's thesis: In silico characterization, recombinant expression and biological functions of TNFRSF10C/TRAILR3 – BIANCA NATALIENE CARVALHO DE CAMARGOS.
  • Master's thesis: Recombinant expression of genetic variants found in patients with Epithelial Ovarian Cancer for functional studies - JÚLIA GUERRA VIEIRA DE SOUSA.

Project Photos and Illustrations:

Figure 1: HEK-293 RAD51B transfection
Figure 1. Transfection of wild-type RAD51B (Vector 1) and RAD51Bc.295delGinsTTO (Vector 2) genes into TOV-21G cells using nanoparticles. A–E: HEK-293 RAD51B expression analysis.
Figure 2: RAD51B protein localization
Figure 2. Subcellular localization of RAD51B protein in transfected TOV-21G cells observed by immunofluorescence microscopy. Images depict nuclear and cytoplasmic distribution.
Figure 3: Nanoparticle-mediated gene delivery
Figure 3. Schematic representation of nanoparticle-mediated gene delivery into TOV-21G cells, showing complex formation and cellular uptake. Adapted from experimental design.
Figure 4: RAD51B protein expression analysis
Figure 4. Western blot analysis showing RAD51B protein levels in TOV-21G cells after transfection with wild-type and mutant constructs.
Figure 5: Nanoparticle uptake in cells
Figure 5. Confocal microscopy images illustrating the uptake of nanoparticles carrying RAD51B constructs into TOV-21G cells. Fluorescent signal indicates successful internalization.
Figure 6: Representation of nanoparticle internalization and endosomal escape
Figure 6. Representation of internalization and endosomal escape promoted by nanoparticles carrying RAD51B constructs. Adapted from experimental design and prepared using BioRender (2024).